TiME Trainee Research

Trainee Publications

2026

• Cholesterol efflux protein, ABCA1, supports anticancer functions of myeloid immune cells

• Clinical trial assessing the use of optical coherence tomography in decision-making for children presenting with acute otitis media

• Enhancing radiomic feature selection in PET-based heterogeneity analysis through tumor-volume-weighted normalization

• In vivo metabolic tagging and targeting of circulating red blood cells

• Investigating Cellular Magnetic Bioeffects Using Two-Channel, Two-Photon Autofluorescence Lifetime Microscopy

• Loss of mast cells and histaminergic signaling link diet to platelet-mediated NETosis and mammary cancer recurrence

• Metabolic Tagging of Tumour Extracellular Vesicles for Targeted Modulation of Dendritic Cells

• Neutrophils exposed to a cholesterol metabolite secrete extracellular vesicles that promote epithelial-mesenchymal transition and stemness in breast cancer cells

• Programmable DNA-Peptide Hybrid Nanostructures for Potent Neutralization of Multiple Influenza a Virus Subtypes

• The Role of the Receptor for Advanced Glycation End-Products in Cancer: Evidence from a Systematic Review and Meta-Analysis

• Self-adjuvanting α-helical polypeptide simultaneously delivers neoantigen mRNAs and activates dendritic cells to eradicate tumors

2025

• Accelerating biopharmaceutical cell line selection with label-free multimodal nonlinear optical microscopy and machine learning
• ACSS2-Mediated Metabolic-Epigenetic Crosstalk Drives Fulvestrant Resistance and Represents a Novel Therapeutic Target
• Cholesterol efflux protein, ABCA1, supports anti-cancer functions of myeloid immune cells
• Cutting-edge approaches to diagnosing and treating limb ischemia in peripheral arterial disease
• A graph-theoretic framework for quantitative analysis of angiogenic networks
• Injectable extracellular vesicle hydrogels with tunable viscoelasticity for depot vaccine
• Label-free hyperspectral multiphoton microscopy

• LRH-1 is a novel regulator of neutrophil-driven immune responses within the tumor microenvironment

• Macroporous hydrogel-based mRNA cancer vaccine for in situ recruitment and modulation of dendritic cells

• Monitoring Molecular Uptake and Cancer Cells’ Response by Development of Quantitative Drug Derivative Probes for Chemical Imaging
• Multiomic Analysis Links Neighborhood Disadvantage to Inflammatory Proteins and Tumorigenic Markers in ER+ Breast Cancer Plasma and Tumor Samples
• Shear-Induced CROSS (Cellular RedOx Spreading Shield) Assembly Sustains Neurotrophic Extracellular Vesicle Production for Functional Neural Networks
• Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
• Tannin-bearing hydrogel adhesives with enhanced mechanical and adhesion strength in response to protein leakage
• Two-photon activation, deactivation, and coherent control of melanopsin in live cells
• Visualizing Cell Structures with Minecraft

2024

• Biodistribution of PET radiotracers in tumor-bearing TRAMP mice administered by retroorbital or jugular vein injections
• Cell Chirality of Micropatterned Endometrial Microvascular Endothelial Cells
• Development of NR0B2 as a therapeutic target for the re-education of tumor associated myeloid cells
• Endometrial decidualization status modulates endometrial microvascular complexity and trophoblast outgrowth in gelatin methacryloyl hydrogels
• Exploring Biliverdin’s Molecular Interactions with Cu- and Fe-Based MOFs: A Unified In Vitro Study with Photoacoustic Analysis
• Exploring the structure, metabolism, and biochemistry of the neuronal microenvironment label-free using fast simultaneous multimodal optical microscopy
• Gelatin methacryloyl biomaterials and strategies for trophoblast research
• In vivo CRISPR base editing for treatment of Huntington’s disease
• Inspiring a convergent engineering approach to measure and model the tissue microenvironment
• Large field-of-view metabolic profiling of murine brain tissue following morphine incubation using label-free multiphoton microscopy
• Modeling the Thermoelastic Sample Response for Subdiffraction Infrared Spectroscopic Imaging
• A molecular toolkit for heterologous protein secretion across Bacteroides species
• NR0B2 re-educates myeloid immune cells to reduce regulatory T cell expansion and progression of breast and other solid tumors
• Neutrophils exposed to a cholesterol metabolite secrete extracellular vesicles that promote epithelial-mesenchymal transition and stemness in breast cancer cells
• Pixelation with Concentration-Encoded Effective Photons for Quantitative Molecular Optical Sectioning Microscopy
• Navigating nonalcoholic fatty liver disease (NAFLD): Exploring the roles of estrogens, pharmacological and medical interventions, and life style
• Predicting DNA damage response in non-small cell lung cancer organoids via simultaneous label-free autofluorescence multiharmonic microscopy
• Primary adipocytes as targetable drug depot to prevent post-surgical cancer recurrence
• Programmable hyperspectral coherent anti-Stokes Raman scattering microscopy
• Receptor for advanced glycation end-products: Biological significance and imaging applications
• Respiratory infection with influenza A virus delays remyelination and alters oligodendrocyte metabolism
• Simultaneous label-free autofluorescence multi-harmonic microscopy driven by the supercontinuum generated from a bulk nonlinear crystal
• Successful dietary changes correlate with weight‐loss outcomes in a new dietary weight‐loss program
• SPLICER: a highly efficient base editing toolbox that enables in vivo therapeutic exon skipping
• Targeting systemic and gut microbial metabolism in ER+ breast cancer
• Temporally optimized and spectrally shaped hyperspectral coherent anti-Stokes Raman scattering microscopy

2023

• A smart coating with integrated physical antimicrobial and strain-mapping functionalities for orthopedic implants
• Automated identification of piglet brain tissue from MRI images using Region-based Convolutional Neural Networks
• CHCHD2 mediates glioblastoma cell proliferation, mitochondrial metabolism, hypoxia‑induced invasion and therapeutic resistance
• Bio-adhesive Macroporous Hydrogels for In Situ Recruitment and Modulation of Dendritic Cells
• Delivering Base Editors In Vivo by Adeno-Associated Virus Vectors
• Development of a cell-free strategy to recover aged skeletal muscle after disuse
• High-Fidelity Micro- and Nano-Scale Infrared Spectroscopic Imaging
• A hydrolyzed lipid blend diet promotes myelination in neonatal piglets in a region and concentration-dependent manner
• Identification of metabolic pathways contributing to ER⁺ breast cancer disparities using a machine-learning pipeline
• Influenza A virus infection disrupts oligodendrocyte homeostasis and alters the myelin lipidome in the adult mouse
• Metabolic tagging of extracellular vesicles and development of enhanced extracellular vesicle based cancer vaccines
• Phase-sensitive detection of anomalous diffusion dynamics in the neuronal membrane induced by ion channel gating
• RAGE as a Novel Biomarker for Prostate Cancer: A Systematic Review and Meta-Analysis
• Re-education of myeloid immune cells to reduce regulatory T cell expansion and impede breast cancer progression
• Siglecs in the Porcine Oviduct and Sialylated Ligands on Sperm: Roles in the Formation of the Sperm Reservoir
• Targeting Duchenne muscular dystrophy by skipping DMD exon 45 with base editors
• T cell-responsive macroporous hydrogels for in situ T cell expansion and enhanced antitumor efficacy

2022

• Chemical imaging of cellular ultrastructure by null-deflection infrared spectroscopic measurements
• Delineating cooperative effects of Notch and biomechanical signals on patterned liver differentiation
• Dietary Cholesterol Causes Inflammatory Imbalance and Exacerbates Morbidity in Mice Infected with Influenza A Virus
• A double crosslinking adhesion mechanism for developing tough hydrogel adhesives
• Effects of Pregnancy-Specific Glycoproteins on Trophoblast Motility in Three-Dimensional Gelatin Hydrogels
• Endocrine-Disrupting Chemicals and Breast Cancer: Disparities in Exposure and Importance of Research Inclusivity
• A feasibility study to test a novel approach to dietary weight loss with a focus on assisting informed decision making in food selection
• In Situ Dendritic Cell Recruitment and T Cell Activation for Cancer Immunotherapy
• Label-free metabolic and structural profiling of dynamic biological samples using multimodal optical microscopy with sensorless adaptive optics
• Matrix Hyaluronic Acid and Hypoxia Influence a CD133⁺ Subset of Patient-Derived Glioblastoma Cells
• Multimodal Handheld Probe for Characterizing Otitis Media — Integrating Raman Spectroscopy and Optical Coherence Tomography
• Optimization of a pericyte therapy to improve muscle recovery after limb immobilization
• Principles for the design of multicellular engineered living systems
• Recyclable cell-surface chemical tags for repetitive cancer targeting
• Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels
• Short FcRn-Binding Peptides Enable Salvage and Transcytosis of scFv Antibody Fragments
• Targeted delivery of liposomal chemoimmunotherapy for cancer treatment
• Targeting Metabolic Adaptations in the Breast Cancer-Liver Metastatic Niche Using Dietary Approaches to Improve Endocrine Therapy Efficacy
• Tracking the binding of multi-functional fluorescent tags for Alzheimer’s disease using quantitative multiphoton microscopy
• Ultra-parallel label-free optophysiology of neural activity

2021

• Dietary tomato inhibits angiogenesis in TRAMP prostate cancer but is not protective with a Western-style diet in this pilot study
• The Cholesterol Metabolite 27HC Increases Secretion of Extracellular Vesicles Which Promote Breast Cancer Progression
• High-speed label-free two-photon fluorescence microscopy of metabolic transients during neuronal activity
• Laminin-111 Improves the Anabolic Response to Mechanical Load in Aged Skeletal Muscle
• Microtissue Geometry and Cell-Generated Forces Drive Patterning of Liver Progenitor Cell Differentiation in 3D
• Thienylpiperidine Donor NIR Xanthene-Based Dye for Photoacoustic Imaging
• Treatment With the CSF1R Antagonist GW2580, Sensitizes Microglia to Reactive Oxygen Species
• Tuning Trophoblast Motility in a Gelatin Hydrogel via Soluble Cues from the Maternal-Fetal Interface

2020

• All-digital histopathology by infrared-optical hybrid microscopy
• Closed-loop atomic force microscopy-infrared spectroscopic imaging for nanoscale molecular characterization
• Cognitive function is preserved in aged mice following long-term β-hydroxy β-methylbutyrate supplementation
• Combined Targeting of Estrogen Receptor Alpha and Exportin 1 in Metastatic Breast Cancers
• A Computational Statistics Approach to Evaluate Blood Biomarkers for Breast Cancer Risk Stratification
• Crosstalk between microglia and patient-derived glioblastoma cells inhibit invasion in a three-dimensional gelatin hydrogel model
• Depixelation and enhancement of fiber bundle images by bundle rotation
• Dietary Tomato or Lycopene Do Not Reduce Castration-Resistant Prostate Cancer Progression in a Murine Model
• Dynamic Tracking Algorithm for Time-Varying Neuronal Network Connectivity using Wide-Field Optical Image Video Sequences
• Electrically stimulated hind limb muscle contractions increase adult hippocampal astrogliogenesis but not neurogenesis or behavioral performance in male C57BL/6J mice
• Freeform Three-Dimensionally Printed Microchannels via Surface-Initiated Photopolymerization Combined with Sacrificial Molding
• Imaging the Landmarks of Vascular Recovery
• Impact of Dietary Tomato on Prostate Carcinogenesis and Progression in Lean and Overweight/Obese TRAMP Mice
• In vivo characterization of minipig skin as a model for dermatological research using multiphoton microscopy
• Living Therapeutics: The Next Frontier of Precision Medicine
• Near-infrared photoactivatable nitric oxide donors with photoacoustic readout
• Pharmacological challenges examining the underlying mechanism of altered response inhibition and attention due to circadian disruption in adult Long-Evans rats
• A Photoactivatable Formaldehyde Donor with Fluorescence Monitoring Reveals Threshold To Arrest Cell Migration
• On-demand Milifluidic Synthesis of Quantum Dots in Digital Droplet Reactors
• Response of neuroglia to hypoxia-induced oxidative stress using enzymatically crosslinked hydrogels
• Simultaneous two-photon activation and imaging of neural activity based on spectral-temporal modulation of supercontinuum light
• Surface Tethering of Inflammation-Modulatory Nanostimulators to Stem Cells for Ischemic Muscle Repair
• Video-rate multimodal multiphoton imaging and three-dimensional characterization of cellular dynamics in wounded skin

2019

• Biodegradable Biliverdin Nanoparticles for Efficient Photoacoustic Imaging
• A Conformationally Restricted Aza-BODIPY Platform for Stimulus-Responsive Probes with Enhanced Photoacoustic Properties
• Can Lycopene Impact the Androgen Axis in Prostate Cancer?: A Systematic Review of Cell Culture and Animal Studies
• Dietary Tomato, but Not Lycopene Supplementation, Impacts Molecular Outcomes of Castration-resistant Prostate Cancer in the TRAMP Model
• Free Fatty Acids Rewire Cancer Metabolism in Obesity-Associated Breast Cancer via Estrogen Receptor and mTOR Signaling
• A gelatin hydrogel to study endometrial angiogenesis and trophoblast invasion
• High Throughput Traction Force Microscopy for Multicellular Islands on Combinatorial Microarrays
• Hyaluronic acid-functionalized gelatin hydrogels reveal extracellular matrix signals temper the efficacy of erlotinib against patient-derived glioblastoma specimens
• The impact of skeletal muscle contraction on CD146⁺Lin⁻ pericytes
• Low fat but not soy protein isolate was an effective intervention to reduce nonalcoholic fatty liver disease progression in C57BL/6J mice: monitored by a novel quantitative ultrasound (QUS) method
• Nanoscale imaging of biological samples with responsivity corrected Atomic Force Microscopy-Infrared (AFM-IR) spectroscopy
• Pericyte transplantation improves skeletal muscle recovery following hindlimb immobilization
• Simultaneous photoacoustic imaging of intravascular and tissue oxygenation
• Tracking metabolic dynamics of apoptosis with high-speed two-photon fluorescence lifetime imaging microscopy

2018

• Acoustogenic Probes: A New Frontier in Photoacoustic Imaging
• CHCHD2 Knockout Alters Mitochondrial Metabolism, Increases Sensitivity to Sulfasalazine, and Decreases Proliferation and Invasive Potential of Glioblastoma Cells Expressing EGFRvIII
• High-speed imaging of transient metabolic dynamics using two-photon fluorescence lifetime imaging microscopy
• Hypoxia activates enhanced invasive potential and endogenous hyaluronic acid production by glioblastoma cells
• The impact of mechanically stimulated muscle-derived stromal cells on aged skeletal muscle
• In situ biodistribution and residency of a topical anti-inflammatory using fluorescence lifetime imaging microscopy
• Influence of Hyaluronic Acid Transitions in Tumor Microenvironment on Glioblastoma Malignancy and Invasive Behavior
• Intra-operative imaging of surgical margins of canine soft tissue sarcoma using optical coherence tomography
• Intraoperative visualization of the tumor microenvironment and quantification of extracellular vesicles by label-free nonlinear imaging
• Near-Infrared Photoactivatable Nitric Oxide Donors with Integrated Photoacoustic Monitoring
• Probe-Sample Interaction-Independent Atomic Force Microscopy-Infrared Spectroscopy: Toward Robust Nanoscale Compositional Mapping
• Processed and raw tomato consumption and risk of prostate cancer: a systematic review and dose–response meta-analysis
• Simultaneous cancer and tumor microenvironment subtyping using confocal infrared microscopy for all-digital molecular histopathology
• Soy Consumption and the Risk of Prostate Cancer: An Updated Systematic Review and Meta-Analysis
• Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling
• Surveillance of Cancer Stem Cell Plasticity Using an Isoform-Selective Fluorescent Probe for Aldehyde Dehydrogenase 1A1

2017

• A bioreducible N-oxide-based probe for photoacoustic imaging of hypoxia
• The Combined Influence of Hydrogel Stiffness and Matrix-Bound Hyaluronic Acid Content on Glioblastoma Invasion
• Intraoperative optical coherence tomography for soft tissue sarcoma differentiation and margin identification
• Investigating the healing mechanisms of an angiogenesis-promoting topical treatment for diabetic wounds using multimodal microscopy
• Label-free in vivo cellular-level detection and imaging of apoptosis
• NitroxylFluor: A Thiol-Based Fluorescent Probe for Live-Cell Imaging of Nitroxyl
• A quantitative framework for the analysis of multimodal optical microscopy images

2016

• Characterization of the structure of low-e substrates and consequences for IR transflection measurements
• Intravascular magnetomotive optical coherence tomography of targeted early-stage atherosclerotic changes in ex vivo hyperlipidemic rabbit aortas
• In vivo evaluation of adipose- and muscle-derived stem cells as a treatment for nonhealing diabetic wounds using multimodal microscopy
• Longitudinal in vivo tracking of adverse effects following topical steroid treatment

2015

• Effect of recombinant interleukin-12 on murine skin regeneration and cell dynamics using in vivo multimodal microscopy
• Enhancement and wavelength-shifted emission of Cerenkov luminescence using multifunctional microspheres
• Fast Infrared Chemical Imaging with a Quantum Cascade Laser
• Magnetomotive Optical Coherence Tomography for the Assessment of Atherosclerotic Lesions Using α_vβ₃ Integrin-Targeted Microspheres
• Non-invasive, real-time reporting drug release in vitro and in vivo

Acknowledgement

NIH Verbiage

Research reported in this publication was supported by the National Institute Of Biomedical Imaging And Bioengineering of the National Institutes of Health under Award Number T32EB019944. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.