Joseph Irudayaraj, PhD, has developed highly sensitive paper based lateral flow technologies for rapid and onsite cervical cancer screening. Utilizing Fluorescence Lifetime Imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS), and Darkfield hyperspectral imaging Dr. Irudayaraj's lab have monitored, in live cells kinase phosphorylation (Abl, Src, Syc, FAK, VEGF, MAPK, Akt etc), mRNA expression at splice variant resolution (BRCA1), and loci-specific epigenetic regulation – methylation and demethylation dynamics. His tools can be applied to assess drug response and toxicity in 2D and 3D cultures and model organisms such as Sea elegans and Zebrafish. At present, his group is integrating the concepts of super-resolution microscopy and deep tissue imaging to perform single molecule experiments in living organisms.
More recently, they have successfully developed nanosize cellulosic oxygen bubbles for hypoxia treatment to address a range of cancers. They have further shown that the oxygen nanobubbles can be guided by ultrasound for enhance localization for targeted therapy and to increase the efficacy of radiation and chemotherapy.